Hexane is reported to be three times more acutely toxic to mice than pentane. Concentrations of 30 000 ppm produce central nervous system (CNS) depression within 30-60 minutes. Concentrations ranging from 35 000 to 40 000 ppm can cause convulsions and death. When mice were exposed to air containing 2.5-3% n-hexane for 4 days, liver enlargement was observed after 24 hours. In another study, mice were exposed to commercial hexane (65-70% n-hexane) 24 hours a day, 6 days a week for one year. Exposure levels range from 100 to 2000 ppm. Animals exposed to 1000 and 2000 ppm n-hexane developed atrophy and degeneration of hind leg muscle fibers.

hexane sds -soluble oil was extracted from the seeds by soaking approximately 5 g of dried Olea europaea seed powder in 50 mL of HPLC-grade hexane in a 100 mL beaker. The contents of the beaker were shaken in an orbital shaker for 15 minutes and allowed to stand overnight. Use Whatman filter paper to filter out the seed cake residue. The seed cake residue was dried in air for 1 hour and then dried in an oven at 105°C for a further 2 hours and cooled to room temperature. The difference in weight of the seed cake is used to calculate the percent composition of the oil.
Hexane-1,3,4,6-tetraketone 58, readily obtained from the bis Claisen condensation of methyl ketone and diethyl oxalate (MeONa in ether) <55JCS1205, 02M641>, with 1,2 -DAB 1a,i and (R ,R)-1,2-DACH 12b react in the same manner as α-diketones in refluxing EtOH, yielding symmetrically substituted quinoxalines 59,60 and asymmetrically substituted quinoxalines Phenoline 61, both with two ketone arms (Scheme 15) <02M641>.

Based on the IR and 1H NMR spectral data, it was shown that among the three possible tautomeric forms (diketimine, dienolimine, and diketoenamine), quinoxaline 59a adopts the diketoenamine form. Of the six possible tautomeric forms of quinoxaline 61 (two each of the ketimine/ketoenamine, hydroxyenimine/ketoenamine, ketoenamine/enolimine forms), Hydroxyenimine/ takes the keto-en-amine form <02M641>. The structures of compounds 59a and 61 were also confirmed by X-ray analysis and deduced from theoretical calculations of possible constrained structures (Fig. 2) <02M641>.